Daigo and activation of natural killer cells and immunoregulation

Professor Noboru Furukawa Laboratory for the use of animal products. Department of Scientific Animal Husbandry of the University of Agriculture in Tokyo.

Brief overview:

The INT method was used to detect an immunostimulating effect on the activity of natural killer cells when ingesting lactic acid bacteria fermentation extract.

The results showed that in healthy mice there was no significant change in the activity of natural killer cells, while the activity of natural killer cells in mice with multiple sarcoma caused by the introduction of B-16 melanoma antigen increased significantly when taking lactic acid bacteria fermentation extract inside compared with other control groups.

Goals:

Natural killer cells function not only causes individual protection of antibodies against viral infections and eliminates tumor cells but also demonstrates the destructive effect of dependent antibodies on virus-infected cells and tumor cells.

Like macrophages (MF), natural killer cells play an important role, acting as a biological defense mechanism before the production of antibodies, in particular, as antitumor effector cells.

When studying the immunostimulating effects of lactic acid fermentation extract, the study focused on the effect of oral administration of lactic acid fermentation extract on natural killer cells.

Method:

Lactic acid bacteria fermentation extract was administered to mice at a dose of 0.2 ml/kg continuously for seven days;

Oral administration to mice with sarcoma implanted with B-16 melanoma began three days after implantation;

The control group consisted of mice who, instead of lactic acid bacteria fermentation extract, were prescribed a dosed saline solution with a phosphate buffer вАФ spleen (effector) cells were incubated with YAC-1 target cells, centrifugally separated, and then tested by the INT method for the activity of natural killer cells against YAC-1 target cells.

Results:

No changes in the activity of natural killer cells were observed in normal mice, regardless of whether they were administered orally a saline solution with a phosphate buffer or an extract of lactic acid fermentation bacteria.

There was an increase in the activity of natural killer cells in mice with sarcoma implanted with B-16 melanoma, compared with the control group of orally administered saline solution with phosphate buffer.

It is believed that the stimulating effect of orally administered Daigo on the activity of natural killer cells becomes apparent only when some forms of stimulus are applied to the immune system of mice, such as the implantation of tumor cells. No excessive activity was detected in the normal immune system.